Angiogenesis as Prospective Molecular Biology Technique for Cancer Study

Esophageal cancer is a disease with increasing frequency in both esophageal squamous cell carcinoma and especially for esophageal adenocarcinoma, according to statistics representing the sixth leading cause of death from malignancy in the world. The study group included 24 cases of squamous cell carcinoma, from which we collected tissue samples histological stained with hematoxylin–eosin and specific staining technique (Verhoeff-van Gieson and Szekely) and then labeled CD34, immunohistochemicaly, in order to mark out the neoangiogenesis. At the end, a micro quantitative study was conducted by using the parallel Weibel grid with a distance between points d = 19.39 m. Vessels and tumor stroma were quantified on a rectangular zone (115.72 μ / 182.45 μ) with 0.0021 mm2 area, on 10 consecutive fields on 5 cases of lesions. Micro vessels were counted in areas with the highest density ("hot spot"), after identifying those areas with low magnification. It was found that the tumor vascular morphology system was different from normal esophageal vascular network. Tumoural vessels are small and they have different shapes or may even be distorted. Microscopic and immunohistochemical studies have shown a correlation between the neoangiogenesis and the inflammatory infiltrate that ensures the progression and the extension of esophageal epithelial tumors.